Expert answer:microbiology lab
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The Genus streptococcus (17)
1. What positive role may be played by the nonpathogenic streptococci
normally found between the teeth and the gingival crevice ?
2. list the precaution that should be taken when obtaining a pharyngeal (throat)
swab.
3. When strep throat is suspected, the physician often will recommend that a
pharyngeal (throat) swab be taken and cultivated on blood agar. Why ?
4. Is the mitis salivarius agar selective or differential medium for streptococci?
Explain.
5. Explain how beta-hemolysis may be distinguished from alpha-hemolysis.
The Genus Staphylococcus (19)
1.What is the relation ship of coagulase activity to the pathogenicity of Staphylococcus aureus?
2.Why are species of Staphylococcus often found as contaminants of ham and ham
products?
3.Why is mannitol salt agar considered a selective as well as a differential medium?
4.Suppose that a coagulase test were performed without a control tube. How would
this omission decrease the reliability of the test?
5.Explain how the ability to produce DNase would benefit the pathogenicity of S.
aureus.
The Enteric Bacteria (20)
1.What conclusion may be drawn if no growth appeared on MacConkey agar and
EMB agar after inoculation of the media and an incubation period ?
2.Could reliable results be obtained if TSI agar were inoculated with a sample
directly from a fecal suspension? Explain
3.Summarize the chemistry of the tests that make up the IMViC series. How might
the IMViC series be a value in the bacteriological analysis of water?
4. List the special procedures that must be used when working with TSI agar, and
indicate why each is necessary.
5.Which procedures in the exercise may be used to isolate Escherichia coli from a
fecal suspension and verify its presence?
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