Expert answer:Object Oriented Model

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biology_bac.xlsx

biology_bacteria.docx

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Independent varaible
(____)
Agar Type
Type of bacteria
Nutrient
Nutrient
Nutrient +antibiotic
Nutrient +antibiotic
blood
blood
(ctrl)
(ctrl)
(ctrl)
Nutrient
Nutrient +antibiotic
blood
a
b
g
a
b
g
a
b
g
# of Colonies
Total # of colonies
Independent varaible
Agar Type
(time)
5s
Nutrient
10s
5s
10s
5s
10s
0s (ctrl)
0s (ctrl)
0s (ctrl)
Type of bacteria
a-irregular, filamentous, opaque
b-small, white, round, entire
Nutrient
a-large, white, round, entire
b-small, white, round, entire
c-orange, round, entire
Nutrient +antibiotic 0
Nutrient +antibiotic a-irregular, filamentous
blood
a
b
g
blood
a
b
g
Nutrient
a-brown, irregular
Nutrient +antibiotic 0
blood
a
b
g
# of Colonies
2
3
5
10
3
0
4
0
2
0
2
5
3
4
0
2
4
1
Total # of colonies
5
18
0
4
2
10
4
0
7
Assignment lab report
Make sure each of the bold parts below are used as section headings in
your lab report. The writing should be in a 12 font double-spaced and
should not be longer than 4 pages (including the tables). Remember to
consult the rubric at the beginning of this manual.
Assignment: Lab Report (75)
Title (make is specific!)
Introduction
In 1 paragraph include the following:


Provide at least three pieces of background information about
bacteria. This information should be relevant to your hypothesis.
Cite outside sources!
Explicitly state the hypothesis and prediction of your
microbiology experiment.

Use one or two sentences to connect your background
information with the importance of your hypothesis and your

prediction for the experiment.
Materials and Methods
• Include table showing how you set up your experiment
In a few sentences describe how sampling was done and the
conditions of incubation
Results (you need figures/tables AND text)




Include the total number of colonies, and total number of different
types of colonies that grew on each of the agar plates if possible.
Distinguish between growth on nutrient agar, blood agar and agar +
tetracycline. Organize your data into tables!!!
Include descriptions comparing your experimental plates to
the control plates
Include descriptions and conclusions about the type of hemolysis you
observed on the blood agar plates.

Include drawings or photos of agar plates (including control plates)

Describe what the bacterial growth looked like on each of the plates
in general terms and in paragraph form
Refer to your drawings, photos and tables in your results text.
Discussion
• In 2-3 sentences, summarize the purpose of the experiment based on
background information that you researched.


Describe how the results supported or refuted your hypothesis and
prediction. Refer to data from the results to do this. You may also
need to explain why results were inconclusive. (1-2 paragraphs).

Describe any problems with observing and quantifying the bacteria
colony growth on the different plates.

How do you think the properties of the food you tested affected this
experiment? What other factors affected the results of this
experiment? If you could repeat your experiment, what would you
change or improve? (three sentences total)

Different species of bacteria have growth requirements that are
specific to their needs. Their needs vary greatly because bacteria grow
everywhere from the hot springs of Yellowstone to the Antarctic.
Based on this idea, do you think the same types of bacteria grew on
the nutrient agar as the blood or tetracycline agar? Why or why not?
Do you have any proof of this from your experiment? Explain in a few
sentences.
In a couple of sentences, write a conclusion about you experiment
and what you learned.
References (Minimum of 3. Cite them in the text, using APA
format.)


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