Expert answer:Final Applied Lab Project
Solved by verified expert:Final Applied Lab Project (1 credit Lab Component) Addresses course outcomes 1-4:recognize and explain how the scientific method is used to solve problemsmake observations and discriminate between scientific and pseudoscientific explanationsweigh evidence and make decisions based on strengths and limitations of scientific knowledge and the scientific methoduse
knowledge of biological principles, the scientific method, and
appropriate technologies to ask relevant questions, develop hypotheses,
design and conduct experiments, interpret results, and draw conclusionsThis
is the culminating lab assessment in BIOL 103. It is designed to assess
your ability to apply the principles of the scientific method.The Effect of low pH on Enzyme ActivityDesign
an experiment in which you will test the effect of an acidic fluid on
enzymatic activity. Recall: enzymes are proteins! To complete this
project, it may be useful for you to first review the Scientific Method
Tutorial, information about pH and enzymes in the text book and course
modules, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you
review Lab 4, you will be reminded that there are several factors that
impact enzymatic activity: pH, temperature, and amount of reagent. It is
OK to use the same enzyme/substrate/method as you did in lab 4 (but
modify the treatment), or you can search on-line to find a different
enzyme/substrate/method for measuring enzyme activity for your project
(include all references).As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid
will modify the outcome of an enzymatic reaction. To successfully
complete this project, you will need to identify the question(s) being
asked in your experiment and the hypothesis that you are testing. In
your experimental design, you must clearly explain what you are doing.
That means that you will need to identify the enzyme, the substrate, the
acidic fluid used as treatment, the control treatment and the method of
measuring enzyme activity, as well as explain your experimental
protocol. You must also thoroughly explain how the acidic fluid impacted
enzyme activity based on the results from your own experiment as well
as knowledge of enzymes and pH from the text book, modules, lab manual
and potentially additional information sources.Hint:
Keep in mind that the acid will change the environmental conditions of
the experiment (for example, a low pH value could change the shape of
the active site on the enzyme protein), without directly participating
in the reaction.Lab Materials You may need all or some of the following, depending on your experimental design:Materials from your lab kit:pH paperhydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the bottle that came with the lab kit)plastic beakers or cupsvinegaryeast (can be purchased at grocery store if more is needed)balloonsplastic bottlemarker for labeling of beakersCell phone/digital camera – pictures requiredYou
may choose to use additional materials (different acidic fluids and/or
different organisms and/or different substrate if you chose an enzyme
other than catalase).Outline:Include the following in your outline:Name of enzyme you will useName of organism (if applicable)The substrate and products in the chemical reactionMethod for measuring enzyme activityTreatment: acidic fluid(s), pH, length of exposure, how you will treat your samplesThe control(s) in the experimentHypothesisHow you will present your data (table and/or type of graph)Anything else you would like to get feedback on before you start your experimentWrite a lab report that includes the following: 1. Title page: descriptive title, your name, course name, semester2.
Introduction: general background information about enzymes and specific
information about your chosen enzyme, the question(s) that you are
asking and a clear hypothesis for your experiment (20 points).3.
Design an experiment. Provide a detailed description of the materials
and methods used to conduct the experiment. Identify control and
experimental samples, as well as independent and dependent variables.
Also include the methods used for data collection and analysis (20
points).4. Conduct the experiment and record your results. Take
picture(s) of your results. What did you observe? Present your data in
table and/or graph format. Remember to label everything and include the
unit of measure with all numbers (20 points)5. Use your knowledge
of enzymes and pH to interpret and discuss your results. It may be
necessary for you to refer to the text book and course modules, lab
manual and/or use additional information resources. What effect does the
acidic treatment have on enzyme activity? Did you get the expected
results? Explain. (20 points)6. State a specific and accurate
conclusion. Is your hypothesis supported by the results? Looking back,
how could you have improved your experiment? (10 points)7. Include a list of references to all information sources used in APA format (5 points).Avoid Plagiarism It is very important to write with your own words.
If you do copy one or two sentences directly (use sparingly), use
quotation marks (“) around the copied text, followed by an in-text
reference. All information sources need to be included in the reference
list and as in-text references. Plagiarism will be reported to the
proper UMUC authority. Guidance on how to avoid plagiarism can be found
here: https://www.umuc.edu/students/academic-integrity/a…SubmissionSubmit your final applied lab project in the assignment folder by the due date specified in the course schedule. GradingYour Outline will be graded based on the following criteria (100 points total): Excellent (90-100%)Good (70-89%) Adequate (60-69%)Unsatisfactory (0-59%)Outline100 points Outline
includes all the required components and planned experiment meets
requirements for the assignment and is clearly and accurately described.
Submitted on time.90-100 pointsOutline missing one or two of the required components, and/or planned experiment does not meet one of requirements for the assignment and/or minor issues with clarity and accuracy.70-89 pointsOutline missing several of the required components, and/or planned experiment does not meet several of the requirements for the assignment and/or major issues with clarity and accuracy.60-69 pointsOutline missing most of the required components and planned experiment does not meet the requirements of the assignment.0-59 pointsYour Final Applied Lab Project will be graded based on the following criteria (100 points total):
Excellent (90-100%)Good (75-89%) Adequate (50-74%)Unsatisfactory (0-49%)Introduction, Question and Hypothesis20 pointsBackground
information about enzymes in general and about specific enzyme used in
project is clearly and accurately written. Questions and hypothesis are
specific, relevant and clearly stated.18-20 pointsBackground
information about enzymes in general and about specific enzyme is
somewhat unclear and/or inaccurate. Questions and hypothesis could be
more specific, relevant and clearly stated.15-17 pointsMissing background information about enzymes in general or about specific enzyme used in project or questions and hypothesis. 10-14 pointMissing background information about enzymes in general and about specific enzyme used in project and questions/hypothesis.0-9 pointsExperimental Design, Materials and Methods20 pointsExperiment
is designed to directly test the hypothesis; description of experiment
is detailed and well written and includes all materials and methods
used.18-20 pointsExperiment is mostly designed to test the hypothesis and/or description of experiment is somewhat inaccurate and/or some information about materials and methods used is missing and/or minor problems with clarity an organization. 15-17 pointsExperiment is barely designed to test the hypothesis and/or description of experiment is inaccurate and/or most of the materials and methods used are missing and/or major problems with clarity an organization. 10-14 pointsExperiment is not designed to test hypothesis and/or description of experiment and materials and methods used are missing. 0-9 pointsResults20 pointsResults are clearly and accurately presented in a table and/or graph and picture(s).18-20 pointsResults are presented, but minor problems with clarity and/or accuracy. 15-17 pointsResults are described, but major problems with clarity and/or accuracy and/or results not presented in table or graph. 10-14 pointsResults are not included0-9 pointsDiscussion of Results20 pointsA clear, accurate and well organized discussion of results that demonstrates good knowledge of enzymes.18-20 pointsMinor problems with clarity, accuracy or organization and/or demonstrates some gaps in knowledge of enzymes. 15-17 pointsMajor problems with clarity, accuracy or organization or demonstrates minimal knowledge of enzymes. 10-14 pointsMissing discussion of results, or major problems with clarity, accuracy or organization and missing understanding of enzymes. 0-9 pointsConclusion10 pointsA
well stated conclusion that is accurate and specific and directly
related to the data collected. Clearly stated whether or not the results
support the hypothesis. Relevant suggestion for improvement of
experiment. 9-10 pointsConclusion stated, but slightly inaccurate, and/or not directly related to data collected and/or suggestion for improvement of experiment not very relevant. 7-8 pointsConclusion stated, but inaccurate and/or barely related to data collected and/or missing suggestion for improvement of experiment. 5-6 pointsMissing conclusion, only suggestion for improvement of experiment. 0-4 pointsCitations5 pointsAll references used are included and in correct APA format, in-text references are included.5 pointsAll references are included, but a few minor mistakes in APA format, and/or missing in-text references.4 pointsMissing one or several references and/or major mistakes in APA format.2-3 pointNo references included or references not in APA format.0-1 pointsGrammar and Spelling 5 points0 – 3 minor spelling and grammatical errors5 pointsBetween 4 and 7 minor spelling and grammatical errors4 pointsBetween 8 and 12 spelling and grammatical errors3 point
How it works
- Paste your instructions in the instructions box. You can also attach an instructions file
- Select the writer category, deadline, education level and review the instructions
- Make a payment for the order to be assignment to a writer
- Download the paper after the writer uploads it
Will the writer plagiarize my essay?
You will get a plagiarism-free paper and you can get an originality report upon request.
Is this service safe?
All the personal information is confidential and we have 100% safe payment methods. We also guarantee good grades